Journal: Frontiers in Oncology
Article Title: RanBP3 Regulates Proliferation, Apoptosis and Chemosensitivity of Chronic Myeloid Leukemia Cells via Mediating SMAD2/3 and ERK1/2 Nuclear Transport
doi: 10.3389/fonc.2021.698410
Figure Lengend Snippet: Expression of RanBP3 is up-regulated in CML. (A) The RanBP3 mRNA level was analyzed in database GSE33075, including 9 healthy individuals and 9 CML patients. After removing the outliers of each group, 7 samples of each group were used for expression analysis. (B) Bone marrow mononuclear cells separated from 3 normal controls and 4 CML patients were used to assess the mRNA levels of RanBP3. (C) The protein levels of RanBP3 were detected in BCR-ABL positive cells K562, K562/G01, KCL22 and BCR-ABL negative cells HL60, THP-1 and NB4. (E) The protein levels of RanBP3 in BCR-ABL transformed mouse cell lines and their corresponding control cells. (D, F) The mRNA levels of RanBP3 correspond to cells in (C) and (E) . (G, H) K562 and K562/G01 cells were treated with various concentrations of IM for 48 hours and the protein (G) and mRNA (H) levels of RanBP3 in these cells were detected. **P < 0.01, ***P < 0.001.
Article Snippet: Mononuclear cells were separated following the instructions of the human bone marrow mononuclear cells (BMMCs) separation regents (Solarbio, China).
Techniques: Expressing, Transformation Assay, Control